What is CCD2?
CCD2 helps you design constructs for protein expression and crystallisation.
1- Give CCD2 the DNA sequence of your ORF.
CCD2 will automatically gather and show information about the properties of your protein.
2- Tell CCD2 where on the protein sequence you want your construct(s) to start and end.
CCD2 will design your PCR primers for you, ready for ordering from your supplier.
3- Order your primers and have a coffee.
Good luck with the cloning :-)
How do I use CCD2?
Just follow the tutorial: